ANTIBACTERIAL AND ANTIBIOFILM ACTIVITY OF MELINJO LEAF WATER EXTRACT AGAINST PSEUDOMONAS AERUGINOSA BACTERIA

Pseudomonas aeruginosa is a multi-drug resistant (MDR) human pathogen and known for their biofilm growth making it difficult to eliminate. This research aims determine the effectiveness of water extract melinjo leaf (Gnetum gnemon L) on inhibition cell attachment, degradation using Crystal Violet (CV) Assay. Reasearch results showed that contains saponin, alkaloid, tanin dan steroidal antibacterial activity antibiofilm against P. aeruginosa. Addition can inhibit attachment 49,80% with concentration 25%, temperature 37,5 oC contact time 45 minutes. With 25% (v/v), 50 °C 3 days, leaves biofilms 43,09% able degrade 43.04% 37.5°C minutes